Figure 1
From: Porcine epidemic diarrhea virus activates PERK-ROS axis to benefit its replication in Vero E6 cells
PEDV infection increased the levels of CHOP and ERO1α expression and cytosolic ROS. Vero E6 cells were infected with PEDV (MOI = 0.001) or mock infected for indicated time points. A Western blotting of CHOP and ERO1α. β-actin was used as loading control. B Ratios of CHOP to β-actin. C Ratios of ERO1α to β-actin. D Cells were measured for changes in ROS level by flow cytometry after incubation with 10 μM DCFH-DA for 30 min at 37 °C. E The changes in cytosolic ROS levels were evaluated according to the mean value of DCFH-DA fluorescence intensity and expressed as fold changes between mock- and PEDV-infected cells. The bar charts in B, C, and E represent mean ± SD of three independent experiments. *, P < 0.05; **, P < 0.01.