Figure 3From: Effector protein Hcp2a of avian pathogenic Escherichia coli interacts with the endoplasmatic reticulum associated RPL23 protein of chicken DF-1 fibroblastsStreptavidin–biotin affinity pull-down and LC–MS/MS. A Interacting proteins in DF-1 cell lysates were pulled down with SA-biotin and SA-biotinylated Hcp2a, respectively, and separated by SDS-PAGE. SA-biotin beads were set as controls. Lane M, protein marker; lane 1, SA-biotin beads; lane 2, SA- biotinylated Hcp2a beads. B Number of proteins identified by LC–MS/MS.Back to article page