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Figure 1 | Veterinary Research

Figure 1

From: Effector protein Hcp2a of avian pathogenic Escherichia coli interacts with the endoplasmatic reticulum associated RPL23 protein of chicken DF-1 fibroblasts

Figure 1

Expression and purification of pET28a-Hcp2a protein. A SDS-PAGE of recombinant bacterial lysates. M, protein maker; 1, uninduced pET28a recombinant bacterial lysate; 2, induced pET28a recombinant bacterial lysate; 3, uninduced pET28a-Hcp2a recombinant bacterial lysate; 4, induced pET28a-Hcp2a recombinant bacterial lysate; 5, pET28a-Hcp2a recombinant bacterial lysate soluble fraction; 6, pET28a-Hcp2a recombinant bacterial lysate insoluble fraction. The arrow indicates Hcp2a protein. B Comparison of pET28a-Hcp2a expression at different temperatures (16, 25, and 37 °C) and different concentrations of IPTG (0.25, 0.5, and 1 μM). Temperature and IPTG concentrations are indicated above the lanes. M, protein maker. The arrow indicates Hcp2a protein. C Different concentrations of imidazole elution of recombinant Hcp2a protein purified by Ni–NTA resin. M, protein maker. 1, 20 mM imidazole; 2, 50 mM imidazole; 3, 100 mM imidazole.

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Veterinary Research

ISSN: 1297-9716