Figure 5

Reassembly of vimentin contributes to the transcription of NOD2 and phosphorylation of p65. A STEC and VIM KO STEC were infected with SS2 (MOI 50) for 2 h, and the transcription of NOD2 was detected by RT-qPCR. B Transcription of NOD2 in the lungs of PBS- or SS2-infected mice was detected by RT-qPCR. C STEC and VIM KO STEC were infected with SS2 (MOI 50) for 2 h, and the expression of phospho-p65 and p65 was analyzed by Western blot. The graph indicated the relative protein level of phospho-p65. D, E STEC pretreated with DMSO or 2.5 μM WFA for 1 h were infected with SS2 (MOI 50) for 2 h, the transcription of NOD2 was determined using RT-qPCR (D), and the expression of phospho-p65 and p65 was analyzed by Western blot (E). The graph indicated the relative protein level of phospho-p65 (E). Data are representative or are presented as the mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.