Table 1 All primer sequences used in this experiment
From: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway
Gene | Primer name | Primer sequence (5ʹ-3ʹ) | Refs. |
|---|---|---|---|
IFNβ | P1 F | CCTCAACCAGATCCAGCATT | [46] [46] |
P1 R | GGATGAGGCTGTGAGAGGAG | ||
IRF7 | P2 F | CGCCACCCGCCTGAAGAAGT | [20] |
P2 R | CTGCCCGAAGCAGAGGAAGAT | [20] | |
RIG-I | P3 F | GCGTACCGCTATAACCCACA | [47] |
P3 R | CCTTGCTGGTTTTGAACGC | [47] | |
β-actin | P4 F | TACGCCAACACGGTGCTG | [46] |
P4 R | GATTCATCATACTCCTGCTTG | [46] | |
pCAGGS-3*Flag-MAVS | P5 F | GATTACAAGGATGACGACGATAAG CTCGAGATGGGCTTCGCGGAGGACAA | New |
P5 R | TTGGCAGAGGGAAAAAGATCTCTA TTTCTGCAGCCGGGCGTACA | New | |
pCAGGS-3*Flag-MDA5 | P6 F | GATTACAAGGATGACGACGATAAG CTCGAG ATG TCGACGGAGTGCCGAGA | New |
P6 R | TTGGCAGAGGGAAAAAGATCTTCA GTCTTCATCACTTGAAGGACAATG | New | |
pCAGGS-RIG-I(N-terminal)-Myc | P7 F | CATCATTTTGGCAAAGAATTCGCCACCATGACGGCGGAGGAGAAG | New |
P7 R | TTGGCAGAGGGAAAAAGATCTTCACAGATCCTCTTCAGAGATGAGTTTCTGCTCTCTTATATCCCACAGTTCACT | New |