Figure 8

The 3CD protein promotes DHAV-1 replication by inhibiting the RIG-I protein.A Cells in the 24-well plate were transfected with different doses of RIG-I (N-terminal)-Flag (the amount transfected into each group was 0.5 µg, and pCAGGS was used to supplement the insufficient group), and the cells were infected with the DHAV-1 CH strain with MOI = 0.5 for 12 h after transfection. Cell samples were collected 36 h after transfection to detect the viral copy number, and the expression of VP3 and RIG-I was detected by Western blotting. B 0.5 µg RIG-I (N-terminal)-Flag was co-transfected with 0.5 µg 3CD-HA or co-transfected with empty plasmid respectively in 12-well plates, and cells were infected with DHAV-1 CH strain with MOI = 0.5 at 12 h. Cell samples were collected at 36 h after transfection to detect the viral copy number, and the expression of VP3, RIG-I, and 3CD was detected by Western blotting. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, compared with the control group.