Figure 6
From: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway
The 3CD protein interferes with the interaction between RIG-I and MAVS. DEFs were co-transfected with 0.8 µg pCAGGS-3CD-HA or empty plasmid, 0.6 µg pCAGGS-3*Flag-MAVS and 0.6 µg pCAGGS-RIG-I (N-terminal)-Myc, respectively, in 6-well plates. Cells were lysed 36 h after transfection and the supernatant was collected for immunoprecipitation assay using an anti-Flag antibody. RIG-I, 3CD, and MAVS were detected by Western blotting using anti-Flag, anti-Myc, and anti-HA antibodies.