Figure 5
From: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway
The 3CD protein interacts with the N-terminal domain of RIG-I.A pCAGGS-3CD-HA and pCAGGS-RIG-I (N-terminal)-Flag were co-transfected or both were co-transfected separately with empty plasmid into 24-well plates (transfection ratio of 1:1), and cell samples were collected 36 h after transfection for indirect immunofluorescence assays to observe the intracellular localization of 3CD and RIG-I. B Cells were seeded in 6-well plates, and 1 µg of pCAGGS-3CD-HA and 1 µg of pCAGGS-RIG-I (N-terminal)-Flag were co-transfected into monolayers. Cells were lysed 36 h after transfection, and lysates were immunoprecipitated with mouse anti-Flag or mouse IgG antibodies and subjected to Western blotting.