Figure 1
From: DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway
Effect of the 3CD protein on the IFNβ signaling pathway.A pCAGGS-3CD-HA and Poly(I:C) were cotransfected into 24-well plate cells (transfection ratio of 1:1), and the cell samples were collected 36 h after transfection and the total RNA was extracted from the samples. Quantitative PCR analysis of the effect of the 3CD protein on poly(I:C)-induced IFN-β and IFN-β-induced MX and OASL mRNA levels. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control group. B 0.6 µg pCAGGS-3CD-HA and 0.4 µg pCAGGS-IFNβ-Flag were co-transfected into cells in 12-well plates, and cell samples were collected 36 h after transfection to detect the expression of IFNβ protein.