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Table 1 Detection of chB19 in the different parts of the swine respiratory tract by virus titration assay in MDCK cells (first line) and RT-qPCR (second line)

From: Infectivity and transmissibility of an avian H3N1 influenza virus in pigs

Tissue

Titer (log10 TCID50/g tissue)

Threshold cycle (Ct)

1 dpi

2 dpi

3 dpi

4 dpi

5 dpi

6 dpi

#1

#2

#3

#4

#5

#6

#7

#8

#9

#10

#11

#12

Nasal mucosa

<a

<

<

<

<

<

<

<

<

<

<

<

respiratory part

33.4

35.9

negb

neg

neg

neg

32.5

neg

neg

36

neg

neg

Nasal mucosa

<

<

<

<

<

<

<

<

<

<

<

<

olfactory partc

neg

neg

neg

neg

neg

neg

neg

neg

neg

34.9

neg

neg

Nasopharynxc

<

<

<

<

<

<

<

<

<

<

<

<

neg

34

neg

neg

neg

neg

36.1

neg

neg

neg

neg

neg

Soft palatec

<

<

<

<

<

<

<

<

<

<

<

<

neg

neg

neg

neg

neg

neg

neg

neg

neg

neg

neg

neg

Tonsil

<

<

<

<

<

<

<

<

<

<

<

<

neg

neg

neg

neg

neg

neg

neg

neg

neg

neg

neg

neg

Proximal trachea

<

<

<

<

<

<

<

<

<

<

<

<

34.3

31.4

29.5

neg

neg

29.1

neg

neg

neg

neg

neg

neg

Distal trachea

<

<

<

<

<

<

<

<

<

<

<

<

32.5

32.8

32.6

neg

36.5

30.5

neg

neg

neg

neg

neg

neg

Lung rightapical + cardiac lobes

4.8

4.7

1.7

<

1.7

<

<

<

<

<

<

<

18

17.5

21.8

22.8

25

25.7

neg

27.2

31.5

29.7

32.3

32.2

Lung rightdiaphragmatic lobe

1.7

<

<

<

<

<

<

<

<

<

<

<

23.8

35

26.4

29.4

neg

neg

neg

32.6

neg

neg

neg

neg

Lung leftapical + cardiac lobes

<

4.3

<

<

<

<

<

<

<

<

<

<

35.6

19.9

25.8

26.3

25.7

28.8

34.8

neg

neg

33

neg

29.1

Lung right

<

<

<

<

<

<

<

<

<

<

<

<

diaphragmatic lobe

33.5

29

35.2

29.5

33.6

neg

neg

36.7

neg

neg

neg

neg

  1. Samples positive by both RT-qPCR and titration are in bold.
  2. a< Below the detection limit (1.7 log TCID50/g for 20% tissue homogenates, 2 log TCID50/g for 10% tissue homogenates).
  3. bResult negative by RT-qPCR (Ct > 37).
  4. c10% tissue homogenates were used for virus titration of these samples, 20% tissue homogenates were used for the remaining samples.