Second passage experiments of chronic wasting disease in transgenic mice overexpressing human prion protein

Table 1 Second passage of CWD-inoculated tg66 mouse brain

Tg66 donor brain^a	Recipient mouse strain	DPI range at euth	TSE suspect^b	RT-QuIC^c
B378-3 717 dpi 19/32 RT-QuIC	Tg66	627–700	5/12	0/12
B378-3 717 dpi 19/32 RT-QuIC	Tg33	552–700	2/12	0/12
B349-1 651 dpi 7/24 RT-QuIC score	Tg66	329–700	3/12	0/12
B349-1 651 dpi 7/24 RT-QuIC score	Tg33	501–700	3/12	0/12
B351-3 662 dpi 4/12 RT-QuIC score	Tg66	532–700	1/12	0/12
B351-3 662 dpi 4/12 RT-QuIC score	Tg33	587–700	2/10	0/10
B377-4 710 dpi 14/24 RT-QuIC score	Tg66	452–700	1/12	0/12
B377-4 710 dpi 14/24 RT-QuIC score	Tg33	378–700	0/11	0/11
B378-4 717 dpi 0/4 RT-QuIC score	Tg66	469–673	1/8	0/8
B378-4 717 dpi 0/4 RT-QuIC score	Tg33	572–700	0/7	0/7
B348-3 635 dpi 0/4 RT-QuIC score	Tg66	657–700	2/8	0/8
B348-3 635 dpi 0/4 RT-QuIC score	Tg33	679–700	0/7	0/7
B354-4 635 dpi 0/4 RT-QuIC score	Tg66	612–700	7/10	0/10
B354-4 635 dpi 0/4 RT-QuIC score	Tg33	553–700	6/10	0/10
B380-1 626 dpi 0/4 RT-QuIC score	Tg66	480–700	1/9	0/9
B380-1 626 dpi 0/4 RT-QuIC score	Tg33	519–700	0/8	0/8
B351-1 662 dpi 0/4 RT-QuIC score	Tg66	463–653	1/10	0/10
B351-1 662 dpi 0/4 RT-QuIC score	Tg33	404–700	1/8	0/8
		Days old
Uninoculated	Tg66	257–735	1/9	0/9
Uninoculated	Tg33	288–572	0/5	0/5

^a Donor brain tissues were obtained from mice previously classified as indeterminate (weak positive) or negative. The dpi and original RT-QuIC scores are provided for reference. Indeterminate mice are in italics.
^b Mice that showed a decrease in body condition (weight loss), weakness and/or neurologic signs (ataxia, gait abnormalities or balance issues) were classified as TSE suspects based on overall assessment of the live mouse.
^c The RT-QuIC assay was used to screen all second passage mice for prion seeding activity. The number of positive mice over the number of mice tested is shown. Mice were scored positive if ≥ 50% of the assay wells were positive. Positive controls were run on each assay plate and were consistently 100% positive (not shown in table).

ISSN: 1297-9716