Table 1 Description and sequences of the oligonucleotides designed to quantify specific ovine genes using real-time PCR.
Genes | Sequences | Amplicon size (bp) | Accession number |
|---|---|---|---|
HSP90AA1 | F 5′-GCCGCCCCTGGAAGGAGACGACGACACG-3′ R 5′-GCCAGGCGAGCCTCGGCAGCGCTCA-3′ | 130 | XM_004017995.3 |
IL-1β | F 5′-GAAGCTGAGGAGCCGTGCCTACGAACA -3′ R 5′-CCAGCACCAGGGATTTTTGCTCTCTGTCC -3′ | 185 | NM_001009465.2 |
IL-4 | F 5′-CATGTGCTTGAACAAATTCCTGGGCGGAC-3′ R 5′-TAGCCTTTCCAAGAGGTCTCTCAGCGTAC-3′ | 124 | NM_001009313.2 |
IFN-γ | F 5′-ACCGATTTCAACTACTCCGGCCTAACTC-3′ R 5′-CAGAAAAACCCAAAAGCACACAGAGCAG-3′ | 97 | NM_001009803.1 |
FoxP3 | F 5′-GCCCATCTGGCTGGGAAGATGGCCCAAACC-3′ R 5′- AGAGGTGCCTCCGCACGGCAAACAGG-3′ | 166 | NM_001144947.1′ |
IL-10 | F 5′-TCAGCCGTGCTCTGTTGCCTGGTCTTCC-3′ R 5′- GGACGTCCCGCAGCATGTGGGGCAG-3′ | 124 | NM_001009327.1 |
TGF-β | F 5′- GGGCTTTCGCCTCAGTGCCCACTGTTC-3′ R 5′- CAGAGGGGTGGCCATGAGGAGCAGG-3′ | 151 | NM_001009400.1 |
TNF-α | F 5′-CCACGCTCTTCTGCCTGCTGCACTTCGG -3′ R 5′-AACGTGGGCTACCGGCTTGTTATTTGAGGC -3 | 146 | NM_001024860.1 |