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Table 2 Monoclonal antibodies used for immunolabelling and combinations (panels) used to phenotype leukocytes

From: Erysipelothrix rhusiopathiae-specific T-cell responses after experimental infection of chickens selectively bred for high and low serum levels of mannose-binding lectin

Abbreviation

Clone

Specificity

Fluorochrome

Panel

1

2

3

CD41/61−Fitc

11C3a

Chicken CD41/61 integrin (GPIIb-IIIa)

Fluoresceinc

X

–

–

KUL01-RPE

KUL01b

Chicken mannose receptor MRC1L-B [36]

R-phycoerythrinc

X

–

–

MHCII−PerCp/Cy5.5

2G11b

Chicken MHC class II

Peridinin chlorophyll-cyanine 5.5d

X

–

–

CD45−PE/Cy7

UM16-6a

Chicken CD45, all isoforms [37]

R-phycoerythrin-cyanine 7d

X

–

–

Bu1-APC/Cy7

AV20b

Chicken Bu-1 (chB6) transmembrane protein

Allophycocyanin-cyanine 7d

X

–

–

CD4−Fitc

CT-4b

Chicken CD4

Fluoresceinc

–

X

–

CD8−RPE

EP42b

β-chain of chicken CD8

R-phycoerythrinc

–

X

X

TCRγ/δ−PerCp/Cy5.5

TCR-1b

Chicken γ/δ T-cell receptor

Peridinin chlorophyll-cyanine 5.5d

–

X

–

CD8α−Cy5

3-298b

α-chain of chicken CD8

Cyanine 5c

–

X

X

CD25−PE/Cy7

AV142a

Chicken CD25, interleukin-2 receptor α-chain

R-phycoerythrin-cyanine 7c

–

X

–

TCRγ/δ−Fitc

TCR-1b

Chicken γ/δ T-cell receptor

Fluoresceinc

–

–

X

CD4−PACBLU

CT-4b

Chicken CD4

Pacific Blueâ„¢c

–

–

X

  1. Panels 1 and 2 were used for whole blood samples, panel 3 was used for activated T-cells in culture.
  2. X: used in panel; –: not used in panel.
  3. aPurchased from Bio-Rad Antibodies.
  4. bPurchased from SouthernBiotech.
  5. cFluorochrome conjugated by manufacturer.
  6. dFluorochrome conjugated using Lightning-Link® conjugation kits (abcam) according to the manufacturer’s protocol.