Figure 5From: Erysipelothrix rhusiopathiae-specific T-cell responses after experimental infection of chickens selectively bred for high and low serum levels of mannose-binding lectin Net proportions of blast transformed cells in different lymphocyte subpopulations upon in vitro ER antigen stimulation for 72 h. Results for cultures of leukocytes obtained from uninfected L10H (uninf; blue bars) or L10L (uninf; green bars) chickens or experimentally ER infected L10H (inf; red bars) or L10L (inf; purple bars) chickens. Chickens were infected on day 0 and spleens (A) and PBMC (B) were collected on day 18 after infection. Values are group means ± 95% CI (9 ≥ n ≤ 12) where non-overlapping CI indicate statistically significant differences. Note that CI for net proportions are asymmetrical, for details see Materials and methods. Lymphocyte subpopulations were identified using monoclonal antibody panel 3 (Table 2) and the gating strategy is described in Additional file 3.Back to article page