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Figure 5 | Veterinary Research

Figure 5

From: Erysipelothrix rhusiopathiae-specific T-cell responses after experimental infection of chickens selectively bred for high and low serum levels of mannose-binding lectin

Figure 5

Net proportions of blast transformed cells in different lymphocyte subpopulations upon in vitro ER antigen stimulation for 72 h. Results for cultures of leukocytes obtained from uninfected L10H (uninf; blue bars) or L10L (uninf; green bars) chickens or experimentally ER infected L10H (inf; red bars) or L10L (inf; purple bars) chickens. Chickens were infected on day 0 and spleens (A) and PBMC (B) were collected on day 18 after infection. Values are group means ± 95% CI (9 ≥ n ≤ 12) where non-overlapping CI indicate statistically significant differences. Note that CI for net proportions are asymmetrical, for details see Materials and methods. Lymphocyte subpopulations were identified using monoclonal antibody panel 3 (Table 2) and the gating strategy is described in Additional file 3.

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