Figure 3

Mapping analysis of three experimental groups. RNA sequencing was employed to analyze all mRNA expression of spleen B cells from mice immunized with or without BP5. A Distribution. The box chart showed the distribution of gene expression levels of three samples. B Saturation. Different colors represented genes classified according to the level of expression. According to the depth of each saturation curve reaching the platform period, the data volume of these experiments reached the needs of further data analysis. C Mapping region. According to the sequence alignment information, the number of sequences aligned to exons, introns and intergenic regions were counted, respectively; a pie chart indicating the proportions was made.