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Table 2 Sequences of the primers used for RT-qPCR

From: DAD3 targets ACE2 to inhibit the MAPK and NF-κB signalling pathways and protect against LPS-induced inflammation in bovine mammary epithelial cells

Gene name

Accession number

Sequence of primers (5′ → 3′)

Product size (bp)

IL-1β

NM_174093.1

F: ATGAAGAGCTGCATCCAACACCTG

R: ACCGACACCACCTGCCTGAAG

110

IL-6

NM_173923.2

F: CACTGACCTGCTGGAGAAGATGC

R: CCGAATAGCTCTCAGGCTGAACTG

115

IL-8

AF232704

F: ATGACTTCCAAGCTGGCTGTTG

R: TTGATA AATTTGGGGTGGAAAG

149

TNF-α

NM_173966.3

F: CTGGCGGAGGAGGTGCTCTC

R: GGAGGAAGGAGAAGAGGCTGAGG

85

ACE2

XM_005228429

F: AGTGGTGGGAGATGAAGCGAGAG

R: GGAACAGACACGCAGGATCACAG

87

ACE

NM_001206668.1

F: GGAGGTGCTGAAGGACATGGTTG

R: CTCGCCGTTCTGCTGGTTCTG

112

MasR

XM_002690376

F: TGGGCTTTGTTGAGAACGGAATCC

R: TGGGTGATGTAGACGGTGAAGGG

82

AT1R

XM_024990377

F: TCGCTTCAGCCAGTGTCAGTTTC

R: ACAATAGCCAGGTAGCGGTCAATG

85

AT2R

XM_024988817

F: TGTATGGCTTGTCTGTCCTCATTGC

R: GGCATACTTCTCAGGTGGGAAAGC

111

β-actin

NM_173979.3

F: CGTCCGTGACATCAAGGAGAAGC

R: GGAACCGCTCATTGCCGATGG

143