Figure 5
From: Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis
The regulatory effect of gga-miR-365-3p/SOCS5 on apoptosis related genes. After 12 h of transfection with synthesized RNA oligonucleotides or plasmids, the CP-II cells were challenged by 100 µL MG-HS (1 × 1010 CCU/mL). RT-qPCR was used to detect the relative expression of BCLXL and BCL2 mRNA; WB was used to detect the protein levels of apoptosis related genes. β-actin was used for normalization. The data are the mean ± S.D. of at least 3 independent experiments.