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Figure 4 | Veterinary Research

Figure 4

From: Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis

Figure 4

The regulatory effect of gga-miR-365-3p on JAK/STAT pathway. After 12 h of transfection with synthesize RNA oligonucleotides, the CP-II cells were challenged by 100 µL MG-HS (1 × 1010 CCU/mL). (A) The mRNA expression of STAT1 and STAT3. (B) The protein expression of STAT1 and STAT3. β-actin served as the loading control. (C) The mRNA expression of SOCS5 was detected by RT-qPCR. GAPDH works as a house-keeping gene. (D) The protein in SOCS5 and JAK/STAT signaling axis were detected by Western-blotting. β-actin was used for normalization. The data are the mean ± S.D. of at least 3 independent experiments.

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