Figure 3
From: Mycoplasma gallisepticum escapes the host immune response via gga-miR-365-3p/SOCS5/STATs axis
gga-miR-365-3p directly targets SOCS5. A, B 24 h after co-transfection of dual-luciferase plasmid and siRNA, luciferase activity was measured using dual-luciferase assays. C Expression levels of SOCS5 in MG-infected CP-II cells. D, E gga-miR-365-3p negatively regulates SOCS5. The expression of SOCS5 in different treatment groups was detected by qPCR or Western-blotting and normalized to GAPDH. Gray values of the indicated protein were measured by Fusion software. The data are the mean ± S.D. of at least 3 independent experiments.