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Figure 3 | Veterinary Research

Figure 3

From: KbvR mutant of Klebsiella pneumoniae affects the synthesis of type 1 fimbriae and provides protection to mice as a live attenuated vaccine

Figure 3

Immune responses to inoculation with 10 CFU or 107 CFU ΔkbvR. Mice were immunized with 105 CFU (n = 10), 107 CFU (n = 5) ΔkbvR or PBS (n = 10) by the subcutaneous route for two doses on days 0 and 14. A Blood was collected on day 28 post-inoculation, and the relative levels of serum IgG antibody against whole cell lysates of NTUH-K2044 were determined by ELISA. The optical density of the horseradish peroxidase reaction was recorded at 450 nm. B Daily weights of mice inoculated with 105 CFU ΔkbvR, 107 CFU ΔkbvR, or PBS over 28 days. C Histopathological examination of the liver, spleen, and lung of 105, 107 ΔkbvR or PBS immunized mice on day 28 post-inoculation. The section was counterstained with hematoxylin and eosin. Scale bars = 100 μm. *: P < 0.05; ****: P < 0.0001.

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