Figure 5

LD increases in macrophages cause increased inflammation and cell damage. RAW264.7 cells were treated with 10 µM C75 for 24 h to inhibit fatty acid synthesis and then incubated with 360 µM oleic acid for 24 h. The cells were then infected with S. uberis in mid-exponential phase (MOI = 10) for 3 h at 37 ℃. A–C Tnf, Il1b, and Il6 mRNA expression. D Nos2 and Cxcl9 mRNA expression relative to inflammation. E, F NAG and LDH activity in RAW2647 cells. All experiments were repeated 3 times. Data are presented as the means ± SEMs (n = 6). *(P < 0.05) = significantly different between the indicated groups by one-way ANOVA.