Figure 4

Effect of LD levels on bacterial load in macrophages. RAW264.7 cells were treated with 10 µM C75 for 24 h to inhibit fatty acid synthesis, incubated with 360 µM oleic acid for 24 h, and then infected with S. uberis in mid-exponential phase (MOI = 10) for 3 h at 37 ℃. A Viable bacteria enumerated as colony-forming units (CFU) on THB agar (left). CFUs were counted by the spread plate method after incubation for 12 h at 37 °C. The efficacy of C75 and oleic acid on the elimination of S. uberis was confirmed by culturing mMEC lysates on THB agar plates. The number of S. uberis colonies in cells (right). Data are presented as the means ± SDs (n = 6). *(P < 0.05) = significantly different between the indicated groups by one-way ANOVA. B, C Representative flow cytometry histogram of the FLI-H channel at 3 hpi in the FITC-d-Lys-S. uberis-infected RAW264.7 cells of the indicated groups. The dotted line indicates background fluorescence in the uninfected cells. Data are presented as the means ± SEMs (n = 6). *(P < 0.05) = significantly different between the indicated groups by one-way ANOVA. Experiments were conducted in triplicate.