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Figure 1 | Veterinary Research

Figure 1

From: FABP4-mediated lipid droplet formation in Streptococcus uberis-infected macrophages supports host defence

Figure 1

S. uberis infection leads to LD increases in macrophages. RAW264.7 cells were infected with S. uberis at an MOI of 10 and incubated at 37 ℃ for 3 h as described in the “Materials and methods”. A BODIPY 493/503 staining for LD visualization (green) increased in S. uberis infection. RAW264.7 cells were fixed with 4% paraformaldehyde and stained with BODIPY. Uninfected cells were incubated under identical conditions. Images are representative of n = 10 samples/group. Scale bar, 20 µm. B Intracellular LD content was evaluated by staining cells (10 000/sample) with BODIPY 493/503, followed by analysis with CellQuest Pro acquisition software and FlowJo software. Data are presented as geometric mean fluorescence intensity (geoMFI). The dotted line indicates background fluorescence in uninfected cells. Experiment B is representative of 3 independent experiments. Data are presented as the means ± SEMs (n = 6, unless otherwise indicated). *(P < 0.05) = significantly different between the indicated groups by unpaired t test.

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