Table 2 Sequences of primer oligonucleotides used in this study
Name | Primer sequence (5’-3’) |
|---|---|
ΔLT-F | ATGAAAAATATAGCTTTCATTTTTTTTATTTTATTAGCATCGCCATTATATGCAAATTGTGTAGGCTGGAGCTGCTTCG |
ΔLT-R | TGTTATATAGGTTCCTAGCATTAGACATGCTTTTAAAGCAAACTAGTTTTTCATACTCATATGAATATCCTCCTTA |
pBR-LT-F | CGGATTGTCTTCTTGTATGAT |
pBR-LT-R | GATCGGTATTGCCTCCTCTAC |
Claudin-1-F | ACCCGCACTACGTCACCTTC |
Claudin-1-R | GGCAGGACACCTGGTCATTG |
Occludin-F | GTGGTAACTTGGAGGCGTCTTC |
Occludin-R | CCGTCGTGTAGTCTGTCTCGTA |
ZO-1-F | AAGGATGTTTACCGTCGCATT |
ZO-1-R | ATTGGACACTGGCTAACTGCT |
ZO-2-F | CAGGCGGGAGATGTCAAGT |
ZO-2-R | ACCATGACCACCCGATCATTT |
ZO-3-F | ATGGCGGTGAGATTCCAGGT |
ZO-3-R | TCTGAATGGCAAAGGAGGAGG |
β-catenin-F | TGGGACCCTTGTTCAGCTTC |
β-catenin-R | AGAGCACAGATGGCAGGTTC |
GAPDH(E)-F | CGTTAAAGGCGCTAACTTCG |
GAPDH(E)-R | ACGGTGGTCATCAGACCTTC |
GAPDH(I)-F | GCACCGTCAAGGCTGAGAAC |
GAPDH(I)-R | ATGGTGGTGAAGACGCCAGT |
CysG-F | GGCAAGGGACGTTTGAAGAC |
CysG-R | CGGCGATGACCAACCAA |
HPRT1-F | CGGCTTGCTCGAGATGTGAT |
HPRT1-R | GCACACAGAGGGCTACGATGT |
TNF-α-F | CGACTCAGTGCCGAGATCAA |
TNF-α-R | CCTGCCCAGATTCAGCAAAG |
IL-6-F | ATGCTTCCAATCTGGGTTCAA |
IL-6-R | CACAAGACCGGTGGTGATTCT |
CXCL-8-F | GCTCTCTGTGAGGCTGCAGTT |
CXCL-8-R | TTTATGCACTGGCATCGAAGTT |
IL-10-F | TGGGTTGCCAAGCCTTGT |
IL-10-R | GCCTTCGGCATTACGTCTTC |
APN-F | ATCGACAGGACTGAGCTGGT |
APN-R | CAAAGCATGGGAAGGATTTC |
faeG-F | GGGAGCTGCTTTCGCTTTTT |
faeG-R | CCTCGGCAAACCACCATAAA |
Flic-F | TGACTCGGGCTGCACGTA |
Flic-R | TTCAGTGGTCTGCGCAACAG |
STa-F | GCTAATGTTGGCAATTTTTATTTCTG |
STa-R | TTTGAAGAGTCAAGTGATTCAGTTGA |
STb-F | TGCCTATGCATCTACACAATCAAAT |
STb-R | ACCTTTTTTACAACTTTCCTTGGCTAT |