Table 1 Strains and plasmids used in this study
Strain/plasmid | Relevant characteristics | Source or reference |
|---|---|---|
DH5α | φ80(lacZ)∆M15 ∆(lacZYA-argF)U169 endA1 recA1 hsdR17(rk−, mk+) | Tsingke |
ETEC C83902 | Wild-type K88ac LT+, STa+, STb+ | [19] |
C83902ΔeltAB | Wild-type K88ac LT−, STa+, STb+ | This study |
C83902ΔeltAB/ pBR-eltAB | C83902ΔeltAB carrying pBR-eltAB plasmid | This study |
C83902ΔfaeG |  C83902 deletion of faeG gene |  This study |
C83902ΔfliC |  C83902 deletion of fliC gene |  This study |
C83902ΔestB |  C83902 deletion of estB gene |  This study |
ETEC 1836-2 | Â Wild-type K88ac EAST1+ | [40] |
ETEC 1836-2/pLT | 1836-2 carrying pBR-eltAB plasmid | This study |
Plasmid | ||
 pBR322-eltAB | pBR322 carrying the full eltAB gene | This study |
 pKD3 | Cm+; Cm cassette template | [20] |
 pKD46 | Amp+, λ-Red recombinase expression | [20] |
 pCP20 | Amp+, Cm+; Flp recombinase expression | [20] |