Figure 5
From: Genotyping and biofilm formation of Mycoplasma hyopneumoniae and their association with virulence
MLST-, P146-, and MLVA-based dendrograms. A MLST- and P146-based dendrograms. The dendrogram was derived from combining individual distance matrices of adk, rpoB, and tpiA sequences. Strains 168, 168 L, NJ, and RM48 show identical ST (green node in B and D), and the remaining six strains were clustered into a different ST. P146 ST are appended to the right and demonstrate consistency in allele differentiation. B Minimal spanning tree calculated with P146 allelic profiles. Samples in the same color belong to the same P146-based genotype. The numbers around the nodes indicate ST derived by MLST. The allelic profiles and P146-based genotyping classified the 10 strains into seven ST. C Dendrogram derived from UPGMA cluster analysis of MLVA profiles obtained using 13 loci. Strains could be delineated into two major clusters, with < 30% similarity. ST and P146-based genotypes are appended to the right and demonstrate consistency in allele differentiation. D Minimal spanning tree of all samples with a complete MLVA profile. Genetic distances among strains NJ, RM48, 168 L, and 168 based on MLVA data were closer than among 7422, XLW-2, and J. Strains LH, 7448, and 232 show relatively large genetic distances with other strains. Samples in one color belong to the same P146-based genotype. MLST data were consistent with P146-based genotyping data.