Figure 10

IRF1-AS positively regulates type I IFN production and ISGS expression during viral infection. A EECs and B GFFs were transfected with si-NC or si-IRF1-AS-1 siRNAs for 24 h and then infected with PPRV at an MOI of 3 for 48 h. The RNA expression levels of IFN-β, ISG15 as well as Mix1 were measured by quantitative RT-PCR. D EECs and E GFFs were transfected with pCDNA3.1 plasmids or pCDNA3.1-IRF1-AS plasmids for 24 h and then infected with PPRV at an MOI of 3 for 48 h. The RNA expression levels of IFN-β, ISG15 as well as Mix1 were measured by quantitative RT-PCR. C EECs were transfected with pCDNA3.1 plasmids or pCDNA3.1-IRF1-AS plasmids for 24 h and then infected with PPRV at an MOI of 3 for 48 h. The protein level of IRF3 and phosphorylation of IRF3 were measured by Western blot assay. F EECs were transfected with si-NC or si-IRF1-AS siRNA for 24 h and then infected with PPRV at an MOI of 3 for 48 h. The protein level of IRF3 and phosphorylation of IRF3 were measured by Western blot assay. The data represent the mean ± SD of three independent experiments. P values were calculated using Student’s t test. An asterisk indicates a comparison with the indicated control. *P < 0.05; **P < 0.01.