Figure 1

PPRV infection activates the innate immune response in EECs. A–F EECs were infected with PPRV at different MOIs for 48 h (A–C), or at an MOI of 3 for the indicated times (D–F), and the protein levels PPRV N expression (A, D), the mRNA levels of ISG15 (B, E) as well as Mix1 (C, F) were measured by Western blot and quantitative RT-PCR assay, respectively. β-actin was used as a loading control in Western blot analysis. The data represent the mean ± SD of three independent experiments. P values were calculated using Student’s t test. An asterisk indicates a comparison with the indicated control. *P < 0.05; **P < 0.01; ***P < 0.001.