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Figure 8 | Veterinary Research

Figure 8

From: A novel C-type lectin from Trichinella spiralis mediates larval invasion of host intestinal epithelial cells

Figure 8

Far-Western blot identification of binding between rTsCTL and IEC proteins. A: SDS‒PAGE analysis of soluble IEC proteins. Lane M: protein marker. Lane 1: IEC proteins. Lane 2: C2C12 proteins. B: Far-Western blot showing the binding of rTsCTL with IEC proteins. Lane M: protein marker; the strips containing IEC proteins (Lanes 1–3) or C2C12 proteins (Lanes 4–6) were incubated with rTsCTL (Lanes 1–6), and rTsCTL binding with IECs was identified by infection serum (Lane 1) and anti-rTsCTL serum (Lane 2) but not by preimmune serum (Lane 3). No binding of rTsCTL with C2C12s was detected with infection serum (Lane 4), anti-rTsCTL serum (Lane 5) or preimmune serum (Lane 6).

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