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Figure 3 | Veterinary Research

Figure 3

From: A novel C-type lectin from Trichinella spiralis mediates larval invasion of host intestinal epithelial cells

Figure 3

Expression and antigenicity analyses of rTsCTL. A: SDS‒PAGE of rTsCTL. Lane M: protein marker; Lane 1: lysate of bacteria carrying pQE-80L/TsCTL prior to induction; Lane 2: lysate of bacteria carrying pQE-80L/TsCTL after induction; Lane 3: purified rTsCTL. B: Western blot analysis of rTsCTL. The lysates of bacteria carrying pQE-80L/TsCTL prior to induction (Lane 1) were not recognized by infection serum, and the lysates of induced bacteria carrying pQE-80L/TsCTL (Lane 2) and purified rTsCTL (Lane 3–6) were recognized with infection serum (Lane 3), anti-His monoclonal antibody (Lane 4) and anti-rTsCTL serum (Lane 5) but not by normal serum (Lane 6).

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Veterinary Research

ISSN: 1297-9716