Figure 12
rTsCTL haemagglutination activity and sugar inhibition. A: Haemagglutination of mouse erythrocytes with various concentrations of rTsCTL. Lanes 1–3: TBS buffer + rTsCTL; Lanes 4–6: TBS buffer + CaCl2 + rTsCTL; Lanes 7–9: TBS buffer + CaCl2 + BSA; Lanes 10–12: TBS buffer + CaCl2. A-F Different concentrations of rTsCTL (0, 25, 50, 100, 200 and 400 μg/mL). B: Inhibition of diverse carbohydrates on rTsCTL haemagglutinating activity to mouse erythrocytes. Lane 1–3: lactose; Lane 4–6: sucrose; Lane 7–9: glucose; Lane 10–12: mannose. A-F are the sugar dilutions at different concentrations of 400, 200, 100, 50, 25 and 0 mM, respectively.