Figure 6

miR126-5p promotes RNA virus replication. A, B DF1 cells were transfected with miR-126-5p mimics or NC, followed by infection with NDV at MOI = 0.01 after 6 h, and intracellular and supernatant relative levels of NP RNA were measured by absolute quantitative real-time PCR detection. C, D DF1 cells were transfected with miR-126-5p inhibitor or NA, followed by infection with NDV at an MOI of 0.01. After 6 h, intracellular and supernatant relative levels of NP RNA were measured by absolute quantitative real-time PCR detection. E, F The effect of miR-126-5p on NDV replication by TCID₅₀. G NDV-GFP or VSV-GFP infected DF1 cells were visualized 48 h post-transfection and post-infection using fluorescence microscopy and representative images are shown for NC, miR-126-5p mimics or inhibitor transfected cells. Scale bar = 100 μm. The data are expressed as the mean ± SD; n = 3. *p < 0.05, **p < 0.01.