Figure 4
From: Chicken miR-126-5p negatively regulates antiviral innate immunity by targeting TRAF3
Inhibition of miR-126-5p promoted chicken antiviral innate immunity during NDV infection. A qPCR analysis of miR‐126‐5p inhibition efficiency, after miR-126-5p inhibitor or NA (Negative control) transfection of DF1 cells at 24 h. B qPCR detection of the RNA levels of innate immune-related genes, including IFNβ, PKR, and MX1 after inhibition of miR-126-5p. C qPCR detection of inflammatory cytokine-related gene mRNA expression, such as IL-Iβ, IL-6, and IL-8 after the inhibition of miR-126-5p. D, E Transfected DF1 cells with miR-126-5p inhibitor or NA, followed by infection NDV for 12 h. D qPCR analysis of miR‐126‐5p inhibition efficiency. E qPCR detection of IFNβ, PKR and MX1 expression levels. F qPCR detection of the expression levels of the inflammatory cytokine-related genes IL-Iβ, IL-6 and IL-8. The data are expressed as the mean ± SD; n = 3. *p < 0.05, **p < 0.01.