Skip to main content
Figure 3 | Veterinary Research

Figure 3

From: Chicken miR-126-5p negatively regulates antiviral innate immunity by targeting TRAF3

Figure 3

Overexpression of miR-126-5p inhibits chicken antiviral innate immunity during NDV infection. A qPCR analysis of miR‐126‐5p overexpression efficiency, after miR-126-5p mimics or NC (Negative control) transfection of DF1 cells at 24 h. B qPCR was used to detect the RNA levels of innate immune-related genes, including IFNβ, PKR, and MX1, after overexpression of miR-126-5p. C qPCR detection of inflammatory cytokine-related gene RNA levels, such as IL-Iβ, IL-6, and IL-8 expression after overexpression of miR-126-5p. D, E Transfection of DF1 cells with miR-126-5p mimics or NC, followed by infection with by NDV for 12 h. D qPCR analysis of miR‐126‐5p overexpression efficiency. E qPCR detection of IFNβ, PKR and MX1 expression levels. F qPCR detection of inflammatory cytokine-related gene IL-Iβ, IL-6 and IL-8 levels. The data are expressed as the mean ± SD; n = 3. *p < 0.05, **p < 0.01.

Back to article page