Figure 5

Swimming motility and c-di-GMP level of bacterial biofilms treated with CRAMP. A represents visual comparison and quantitative determination of the swimming motility of PAO1 biofilms (3 days) treated with CRAMP. Dispersal cells were inoculated into the plates with 0.5% agar and grown for 24 h. Swimming motility was measured by diffusion diameter on the agar surface. B represents the c-di-GMP level of PAO1 biofilms treated with CRAMP measured using the pcdrA::lux reporter. Bioluminescence and OD were measured using a plate reader. The c-di-GMP level was calculated as Lux/OD600. C represents the change in log₁₀ of PAO1 in different states (biofilm cells or dispersion cells) compared with the control group after treatment with different concentrations of CRAMP. The blue colour represents the change in the number of dispersed cells in the upper suspension compared with that of the control group, and the grey colour represents the change in the number of biofilm cells compared with that of the control group. An unpaired t test (two-tailed) was used to measure statistical significance. *P < 0.05, **P < 0.01 compared with the control group.