Figure 4
From: Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP
The contents of EPS, alginate, and rhamnolipid in P. aeruginosa PAO1 biofilms treated with CRAMP. The bacterial suspension was cultured in 6-well plates for 3 days, and the biofilm was treated with CRAMP for 1 h. The biofilm was collected with a cell scraper, and the contents of EPS (total polysaccharides of biofilms + total protein of biofilms), alginate, and rhamnolipid in the biofilms were detected. A–C represent the percentages of EPS, alginate, and rhamnolipid in the PAO1 biofilms treated with CRAMP, respectively. The number of biofilm bacteria in the CRAMP and control groups was normalized to the same level, and the control group was considered 100% for calculation. An unpaired t test (two-tailed) was used to measure statistical significance. *P < 0.05, **P < 0.01 compared with the control group.