Figure 1
From: Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP
Anti-biofilm activity of CRAMP against P. aeruginosa PAO1. The PAO1 suspension was adjusted to OD600 = 0.1 with MH broth and diluted 100 times. The suspension was cultured in different culture plates at 37 °C and then treated with antibiotics or CRAMP for 1 h. The biomass was detected by crystal violet (CV) staining, and the viable bacteria of the biofilm were detected by colony counting (CC). A Represents the biomass of PAO1 biofilm (1 day) treated with CRAMP, LL-37, gentamicin (GEN), and ciprofloxacin (CIP) in 96-well plates detected by CV. B Represents the biomass and viable bacteria, in which the PAO1 biofilm (3 days) was treated with CRAMP in 6-well plates and cell culture flasks. An unpaired t test (two-tailed) was used to measure statistical significance. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with the control group.