Figure 7

Expression of humoral and cytokine responses. A. Total IgG antibody response. Mouse serum was collected, and total IgG antibodies against the recombinant protein were assessed at 14 and 21 days post-inoculation. The level of IgG was compared with that of the PBS control group. B. Expression of cytokine genes. The spleen was harvested at 3 days post-inoculation, and mRNA was extracted to determine the expression levels of the inflammatory cytokines TNF-α, IFN-γ, and IL-1β by quantitative real-time PCR. A significant difference against the WT strain for the expression of genes was measured and analysed by multiple unpaired t tests and is presented as p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001.