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Figure 5 | Veterinary Research

Figure 5

From: Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems

Figure 5

Evaluation and confinement of bacterial load in different internal organs post-inoculation. Mice were administered Salmonella by the IM and PO routes. A. Bacterial load. The spleen, liver, lung, and brain were aseptically collected. Samples were homogenized in PBS, and the bacterial load was quantified in Salmonella selective media, BGA. On 4 dpi, all JOL401 IM-inoculated mice died, and the bacterial load was not analysed. For both JOL2782 and JOL2837, bacteria were not recovered from the tested organs at 14 and 21 dpi. Data were analysed by multiple unpaired t tests and are presented as *p < 0.05, **p < 0.01, and ***p < 0.001. B. Immunohistochemical staining of tissues (IHC). Distribution and delivery of Salmonella in mouse organs were evaluated by IHC staining of tissue sections at 3 dpi. Brown spot regions indicated by black arrowheads represent the bacteria inhabiting the tissues. Images were obtained at 100X magnification, and inset images were obtained at 400X magnification. The scale bar indicates 200 μm.

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