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Figure 3 | Veterinary Research

Figure 3

From: Safety assessment of compliant, highly invasive, lipid A-altered, O-antigen-defected Salmonella strains as prospective vaccine delivery systems

Figure 3

Characterization of protein expression in RAW 264.7 cells. Murine macrophage RAW 264.7 cells were infected with the vaccine strains JOL2782 and JOL2837 or the control. Protein expression was determined by A. Western blots and IFA. A. A Western blot image demonstrates the band representing the expression of the recombinant protein (22 kDa) in RAW264.7 cells. B. IFA was performed using rabbit antisera against the recombinant protein. Bright green fluorescence in the JOL2782- and JOL2837-infected cells indicates protein expression. Blue represents the nucleus stained by DAPI. No fluorescence was detected in the control.

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