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Figure 1 | Veterinary Research

Figure 1

From: The important role of NLRP6 inflammasome in Pasteurella multocida infection

Figure 1

NLRP6 protects the lung from P. multocida infection. WT and Nlrp6−/− mice were intranasally infected with 20 µL P. multocida (1000 CFU) and the same volume of sterilized PBS were used as control. Lungs were collected after 48 h infection for hematoxylin and eosin staining (original magnification × 100 and × 400). A Pictures are representative of three mice from each group of mice (n = 3). B The lungs were homogenized and colony counting was performed after 48 h infection (n = 5). Flow cytometry was used to analyze the number of neutrophils (C) and macrophages after 24 h infection (D) (n = 5–8). Lung homogenates were collected after 3 h, 6 h, 9 h, 24 h infection, and tissue RNA was extracted to detect mRNA expression of cxcl1 (E) and cxcl2 (F) mRNA (n = 5 in each time point). The student t-test was used to analyze the statistical differences for comparisons between two groups. Statistical significance was determined as p value, *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significance.

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