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Figure 2 | Veterinary Research

Figure 2

From: Comprehensive evaluation of the safety and immunogenicity of a gene-deleted variant pseudorabies virus attenuated vaccine

Figure 2

Characterization of the gene-deleted PRV GX-ΔTK/IES recombinant virus. A Crystal violet staining of PK15 cells at 96 h after infection with PRV GX-ΔTK/IES and parental PRV GX. A cytopathic effect with virus plaques was clearly visible. B One-step growth curves of gene-deleted PRV GX-ΔTK/IES and parental PRV GX in PK15 cells. Confluent monolayers of PK15 cells were infected at a multiplicity of infection (MOI) of 0.1, and total freeze/thaw lysates were titrated at 4, 8, 12, 24, 36, and 48 h after infection. Virus titres are described as TCID50/mL in log 10 steps.

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