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Table 2 Products used to stimulate the immortalised buccal epithelial cells

From: Immortalised canine buccal epithelial cells’ CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol

Group of products

Name

Working concentration

Supplier

Allergen extracts

Dermatophagoides farinae (XPB81D3A2.5, Lot: 307,244)

20 µg/mL

(2.1 µg/mL Der f 1)

Greer laboratories, Lenoir, NC, USA

Dermatophagoides pteronyssinus

(XPB82D3A2.5, Lot: 346,230)

20 µg/mL

(0.58 µg/mL Der p 1)

Alternaria alternata

(XPM1D3A2.5, Lot: 312,142)

20 µg/mL

Birch (Betula pendula)

(XP527D3A2.5, Lot: 350,600)

20 µg/mL

Timothy grass (Phleum pratense)

(XP28D3A2.5, Lot: 305,467)

20 µg/mL

TLR ligands

Heat-killed Listeria monocytogenes (HKLM) (TLR2)

108 cells/mL

Invivogen, San Diego, CA, USA

Pam3CSK4 (TLR1/2)

1 µg/mL

FSL-1 (TLR2/6)

1 µg/mL

Polyinosinic:polycytidylic acid high molecular weight

(poly I:C HMW) (TLR3)

10 µg/mL

Polyinosinic:polycytidylic acid low molecular weight

(poly I:C LMW) (TLR3)

10 µg/mL

LPS (Escherichia coli K12) (TLR4)

10 µg/mL

Flagellin (Salmonella Typhimurium) (TLR5)

1 µg/mL

Imiquimod (TLR7)

10 µg/mL

ssRNA 40 (TLR8)

10 µg/mL

ODN 2006 (TLR9)

5 µM

Recombinant cytokines

Canine IFN-γ

10 µg/mL

R&D Systems, Minneapolis, MN, USA

Canine IL-10

10 µg/mL

Canine IL-17A

10 µg/mL

Canine IL-4

10 µg/mL

Canine IL-6

10 µg/mL

Human TGF-β1

10 µg/mL

Vitamins

Calcitriol

1 µM

Merck, Burlington, MA, USA

All-trans-retinoic acid

10 µM

9-cis-retinoic acid

10 µM

  1. If the product is an agonist of a specific receptor, the receptor is underlined. TLR = Toll-like receptor.