Immortalised canine buccal epithelial cells’ CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol

Table 1 Antibodies used for immunohistochemistry and flow cytometry

	Antibody	Clone	Working concentration	Supplier
Primary antibody	Rat anti-human CD49f	NKI-GoH3	10 µg/mL	Thermofisher Scientific, Waltham, MA, USA
Primary antibody	Mouse anti-rabbit cytokeratin 3/2p	AE5	10 µg/mL	Santa Cruz Biotechnology, Dallas, TX, USA
Isotype control	Rat IgG2a anti-KLH	RTK2758	10 µg/mL	Biolegend, San Diego, CA, USA
Isotype control	Mouse IgG1 anti-F4	IMM01	10 µg/mL	In-house hybridoma
Secondary antibody	FITC-conjugated goat anti-mouse IgG	Polyclonal	5 µg/mL	Biolegend, San Diego, CA, USA
Secondary antibody	FITC-conjugated goat anti-rat IgG	Polyclonal	5 µg/mL	Biolegend, San Diego, CA, USA

ISSN: 1297-9716