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Figure 2 | Veterinary Research

Figure 2

From: Baicalin acts as an adjuvant to potentiate the activity of azithromycin against Staphylococcus saprophyticus biofilm: an in vitro, in vivo, and molecular study

Figure 2

Baicalin enhanced the bactericidal effect of azithromycin on preformed biofilms. Bac: baicalin; Azm: azithromycin. A Confocal laser scanning microscopy images of azithromycin- and baicalin-treated preformed MDRSS biofilms. The biofilms were stained with a Live/Dead BacLight™ kit. Live and dead cells were stained green and red, respectively. Yellow indicates the superposition of green and red. White scale bars = 200 μm. The B graph represents the means of the log CFU ± SD obtained by viable count. C The ratio of PI fluorescence intensity/total fluorescence intensity. All the values were obtained in triplicate in three independent experiments. In the same index, the graph bars in a–c with different letters on top represent statistically significant results (p < 0.05), whereas the bars labelled with the same letter correspond to results with no statistically significant differences (p > 0.05).

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