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Figure 2 | Veterinary Research

Figure 2

From: Chicken cathelicidin-2 promotes NLRP3 inflammasome activation in macrophages

Figure 2

CATH-2 induces NLRP3 activation via ASC oligomerization and caspase-1 activation in LPS-primed macrophages. Cells from WT, Nlrp3−/−, Asc−/−, Casp1−/− mice were primed with LPS for 3 h and then CATH-2 (5 μM) and ATP (1.5 mg/mL) as positive control were added for an additional 3 h or 21 h. After 24 h, cell supernatants and lysates were collected for different assays. ELISA was used to determine cytokine secretion of IL-1β (A) and TNF-α (B); A Representative image of the Western blot assay is presented to show protein expression including IL-1β, caspase-1, pro-IL-1β, pro-caspase-1 and β-action (C) as well as ASC protein expression (D); qPCR was used to measure mRNA expression of IL-1β (E) and NLRP3 (F). Data in A, B, D, F are represented as mean ± SEM of three independent experiments from triplicate samples per experiment. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.005.

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