Table 2 List of primers and corresponding sequences used in this study
Primer | Sequence (5'-3') | Description |
|---|---|---|
ISG15-F | GCAAAGCTTCAGAGACCCAC | qPCR for ISG15 mRNA |
ISG15-R | GCCAGACCTCATAGGCGTTG | |
pGM-CSF-F2 | ATGCCATCAAAGAAGCCCTGA | qPCR for pGM-CSF mRNA |
pGM-CSF-R2 | GCTTGTACAGGTTCAGGCGA | |
CCL2-F | GCAAGTGTCCTAAAGAAGCAGTG | qPCR for CCL2 mRNA |
CCL2-R | TCCAGGTGGCTTATGGAGTC | |
CXCL10-F | TGCCCACATGTTGAGATCAT | qPCR for CXCL10 mRNA |
CXCL10-R | CGGCCCATCCTTATCAGTAG | |
GAPDH-F | CCTTCCGTGTCCCTACTGCCAAC | qPCR for GAPDH mRNA |
GAPDH-R | GACGCCTGCTTCACCACCTTCT | |
iNOS-F | GCACCTGCGTTATGCCACCAAC | qPCR for iNOS mRNA |
iNOS-R | TGAGCTGAGCGTTCCAGACCC | |
IFN-γ-F | TCACTGATGGCTTTGCGCTG | qPCR for IFN-γ mRNA |
IFN-γ-R | AGAGCATGATCCGAGACGTG | |
TNF-α-F | AGAGCATGATCCGAGACGTG | qPCR for TNF-α mRNA |
TNF-α-F | CAGTAGGCAGAAGAGCGTGG | |
IL-6-F | ACAAAGCCACCACCCCTAAC | qPCR for IL-6 mRNA |
IL-6-R | CGTGGACGGCATCAATCTCA | |
CD163-F | TCCTTGTGGGATTGTCCTGC | qPCR for CD163 mRNA |
CD163-F | AGGGATTCTCGGCTCTTTGC | |
IL-4-F | CTTCGGCACATCTACAGACACC | qPCR for IL-4 mRNA |
IL-4-R | CTTCATAATCGTCTTTAGCCTTTCC | |
IL-8F | TCCTGCTTTCTGCAGCTCTC | qPCR for IL-8 mRNA |
IL-8R | GGGTGGAAAGGTGTGGAATG | |
IL-13-F | GGTCAATATCACCCAGAACCAGAAG | qPCR for IL-13 mRNA |
IL-13-R | TGCAGTCGGAGATGTTGATGAGG | |
IL-12-F | TACCACTTGAACTAGCCACGAAT | qPCR for IL-12 mRNA |
IL-12-R | CTAAGGCACAGGGTTGTCATAAA | |
CCL17-F | ATGCAGCTCGAGGAACCAAC | qPCR for CCL17 mRNA |
CCL17-R | GTCACAAGCACAATGGCGTC | |
IL-1β-F | GACCCCAAAAGATACCCAAA | qPCR for IL-1β mRNA |
IL-1β-R | TCTGCTTGAGAGGTGCTGATG | |
TGF-β-F | TCCAAGGACCCTTCTCGGAT | qPCR for TGF-β mRNA |
TGF-β-R | AAAAACCGAGATGGGCGAGA | |
MGL-1-F | ACTTCTCCGGCATGGTTCTG | qPCR for MGL-1 mRNA |
MGL-1-R | GTTGAGCACTTTCGCAGCAA | |
IRF-4-F | CCGTCATTAGTGCGTCAGTTCT | qPCR for IRF-4 mRNA |
IRF-4-R | TTGCAGCCCACAAAAAGCA | |
IL-10-F | CGGCGCTGTCATCAATTTCTG | qPCR for IL-10 mRNA |
IL-10-R | CCCCTCTCTTGGAGCTTGCTA |