Figure 5
From: The DHAV-1 protein VP1 interacts with PI3KC3 to induce autophagy through the PI3KC3 complex
The VP1 protein interacts with the PI3KC3 protein. A VP1-Flag and PI3KC3-HA plasmids were co-transfected into DEFs, cell lysates were subjected to Co-IP assays with a Flag-tagged antibody or normal IgG as negative controls, and co-immunoprecipitated proteins were detected by western blot analysis. B VP1-Flag and PI3KC3-HA plasmids were co-transfected into DEFs, cell lysates were subjected to a Co-IP assay with an HA-labelled antibody or normal IgG as negative controls, and co-immunoprecipitated proteins were detected by western blot analysis. C DEFs were treated with 3-MA, and transformation of the PI3KC3 complex and LC3 were detected by western blotting. D Grey value analysis of transformation changes of the PI3KC3 complex and LC3 after 3-MA drug treatment; ns indicates no difference, *P < 0.05, **P < 0.01.