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Figure 3 | Veterinary Research

Figure 3

From: The DHAV-1 protein VP1 interacts with PI3KC3 to induce autophagy through the PI3KC3 complex

Figure 3

The DHAV-1 structural protein promotes induction of autophagy but not intact autophagic flux in DEFs. A VP0, VP3 and VP1 proteins were expressed in DEFs, and expression of the SQSTM1/p62 protein and transformation of the LC3 protein were detected by western blot. B Grey value analysis of changes in LC3 conversion levels after VP0, VP3 and VP1 proteins were expressed in DEFs, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. C GFP-LC3 was co-transfected with the VP0, VP3 and VP1 protein plasmids into DEFs. After samples were collected and fixed, protein expression levels of VP0, VP3 and VP1 were detected by labelling antibodies, and punctate aggregation of LC3 was observed by microscopy. D Using the pmCherry-GFP-LC3 dual-fluorescence system, the VP0, VP3 and VP1 proteins were expressed in DEFs, and green fluorescence and red fluorescence were observed under a microscope. E Grey value analysis of changes in SQSTM1/p62 protein levels after expression of VP0, VP3 and VP1 proteins in DEFs; ns indicates no difference, **P < 0.01.

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