Potential genetic robustness of Prnp and Sprn double knockout mouse embryos towards ShRNA-lentiviral inoculation

Table 2 Differentially expressed genes between Prnp^0/0Sprn^0/0 and WT E6.5 mouse embryos.

Gene name	Description	NCBI gene	Log fold change	Adjusted P-value	Embryo ectoderm	Embryo endoderm	Embryo mesenchyme	Extraembryonic component	RT-qPCR Prnp^0/0 Sprn^0/0 E6.5	RT-qPCR WT E6.5	RT-qPCR P value
Spint1	Serine protease inhibitor, Kunitz type 1	20732	1.599051	1.96414E−08	✓	✓	✓	✓	29.56 ± 2.1	25.88 ± 4.5	0.126
Gm30906	Long non-coding RNA	102632964	− 1.477668	1.96414E−08	✓		✓
Scg5	Secretogranin V, secreted chaperone protein	20394	−1.334427	4.2595E−08	✓		✓
Spg11	Spatacsin vesicle trafficking associated	214585	−1.081024	2.05026E−06	✓		✓
Cds2	CDP-diacylglycerol synthase 2	110911	1.297156	2.42507E−06	✓		✓	✓	50.87 ± 5.5	26.31 ± 4.8	0.001
Jmjd7	Jumonji domain containing 7	433466	−1.479236	1.31005E−05	✓		✓
Ada	Adenosine deaminase	11486	−2.117557	4.32324E−05	✓		✓	✓	10.53 ± 1.1	16.08 ± 4.3	0.043
AK148702		RIKEN clone 7120437D13 (MGI:3537747)	2.819679	0.004937838	–	–	–	–
Cplx2	Complexin 2	12890	2.616736	0.010457547	✓		✓
Gm10734		RIKEN clone I530011G18 (MGI:3565867)	−1.104109	0.010457547	✓		✓
Sdc4	Syndecan 4	20971	−1.157117	0.021156447	✓		✓

Results are shown for significantly differentially expressed genes (FDR < 0.05, absolute log fold change ≥ 1). Checkmarks for each gene represent reported expression in embryo ectoderm, embryo endoderm, embryo mesenchyme, and extraembryonic component [20]. Blank spaces and dashes represent unreported expression and no available data, respectively. Normalized RT-qPCR expression levels are reported in each group for the three tested genes (mean ± standard errors) with the associated P-value from a two-sample Student’s t-test.

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